• Semen Analysis

    Laboratory measurements of sperm quality which concern macroscopic (sperm volume, liquefaction, viscosity) and microscopic (sperm number, motility, vitality, morphology, maturation, coagulations, cell types) parameters .Semen analysis is a standard procedure followed in order to determine the proper function of testes and secretory activities of the glands.

    • Sperm Vitality
      • Eosin-nigrosin [WHO]
      • Hypo-osmotic swelling [WHO]
    • Sperm Morphology
      • Papanicolaou Staining [WHO]
      • Shorr Staining [WHO]
    • Sperm Maturation 
      • Aniline Sulfate
    • Sperm Number
    • Sperm Motility
    • Sperm Volume
    • Agglutinations
    • White/Red Blood Cells
    • Liquefaction
    • Viscosity
  • Sperm DNA Fragmentation test

    Fragmentation of sperm DNA is a factor that indicates a male infertility issue, since spermatozoa with shredded DNA are not capable of proper fertilization, and if this occurs, there is a high risk of forming a pathologic embryo or affecting a blastocyst’s implantation. This test investigates the integrity of sperm DNA revealing the amount of DNA damage that may affect a couple’s fertility.

    • Apoalert DNA  fragmentation Assay Kit (CLONTECH)
    • Halosperm Kit (HALOTECH)
    • Fluorescent Staining : acridine orange stain
    • Tunel [WHO]
  • Mitochondrial Assessment

    A research procedure evaluating mitochondrial activity. Mitochondrial potential indicates sperm functionality as correlated with sperm motility and capacity of fertilization.

    Mitotracker Mitochondrial Selective Probes

  • Nucleus DNA Concentration: fluorescent Staining

    Fluorescence microscopy being used to optimize nucleus DNA density as spermatozoa’s chromatin is normally, highly coiled.

    • Hoechst Stain
    • Dapi Stain
  • Acrosome Reaction Assessment

    Α research procedure which investigates by mimicry, the capability of spermatozoa’s membrane to fuse with the plasma membrane of the oocyte.

    • Aric test (acrosome reaction after hormone challenge test) [WHO]
    • Sperm Hyalouronan binding assay
  • Semen Preparation

    A procedure which separates spermatozoa from seminal plasma for both diagnostic tests and therapeutic purposes. The most suitable technique according to the nature of the sperm is chosen, in order to collect a high percentage of morphologically normal and motile cells, free from debris, non germ cells and dead spermatozoa. After the selection is completed, the appropriate ART (assisted reproductive technology) is used.

    • Discontinuous Density Gradient [WHO]
    • Direct Swim UP [WHO]
    • Discontinuous Density Gradient and Swim UP
    • Preparation of Testicular and Epididymidal spermatozoa [WHO]
  • Anti-sperm Antibody Test

    A test which investigates the presence of antibodies in sperm reacting with antigens on spermatozoa. These antibodies are considered as an immunological infertility factor.

  • Cryopreservation (Sperm Freezing)

    The correct freezing procedure using the “Glycerol Egg Yolk Citrate” cryoprotectant minimizes the intracellular ice crystal formation. As a result, the survival percentage after thawing is the best possible.

  • Micro­surgery TESA , MESA , PESA

    Some men face problems that involve a complete inability to produce an ejaculate with sperm for fertilization processes. This is called azoospermia (obstructive or non obstructive) and can be caused by several reasons that can be genetic or acquired. In these cases surgical extraction of sperm is a viable alternative.

    The removal of the sperm can be accomplished, under general anesthesia, by needle aspiration or by surgical means from the epididimys or directly from the testicular tissue. The sperm found can then be used for ICSI. In greater quantity of sperm is important to be cryopreserved for future use.